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Nucleic Acid Purification & Analysis> | … coupled device camera. Fragment sizes were estimated using a 100 bp DNA ladder (M106R; Genscript Biotech Corporation, Nanjing, China). Results Expression of Epac1 and Rap1a mRNA in MII‑stage oocytes. The expression … | Get A Quote |
Cyclic adenosine monophosphate (cAMP) is an important secondary messenger that has long been recognized to control the initiation of meiosis through the activation of protein kinase A (PKA) in mammalian oocytes. However, PKA is not the only target for cAMP. Recent studies on cAMP-dependent and PKA-independent pathways suggest that Ras-related protein-1 (Rap1) is activated through its cAMP-responsive guanine exchange factors (cAMP-GEFs), which comprises the involvement of exchange proteins directly activated by cAMP (Epac) in various cellular processes. The aim of the present study was to investigate the possible implication of a cAMP/Epac/Rap1 pathway in mouse oocytes and embryos. Reverse transcription poly... More